[Eagle Biosciences] 11,12 DHET ELISA Assay Kit

어스바이오는 Eagle Biosciences 전문 취급점으로서 모든 제품을 전문적으로 취급 및 공급하고 있습니다.
Eagle Biosciences의 Immunology Assay Kits 제품을 소개드립니다.
 

 

Description:

11, 12-DHET ELISA Assay kit는 enzyme linked immunoassay (ELISA)으로 생물학적 샘플에서 11, 12-DHET를 정량적으로 측정하기 위한 것입니다.  Eagle Biosciences의 11, 12-DHET ELISA Assay kit는 연구용으로만 사용되며 진단 절차에 사용해서는 안 됩니다.

 

• Size: 1*96 wells
• Sensitivity: 0.01 ng/ml
• Dynamic Range: 0.01 – 1000 ng/ml
• Incubation Time: 2.5 hours
• Sample Type: Serum, Plasma, Tissue, Biological Fluids
• Sample Size: 100 µl

Assay Procedure:

• Load 200 microliters of Sample Dilution Buffer into the blank (BL) wells and 100 microliters of Sample Dilution Buffer into the maximum binding (BO) wells.
• Load 100 microliters of each of the standards into the appropriate wells.
• Load 100 microliters of each of the samples into the appropriate wells.
• Load 100 microliters of the diluted 11,12-DHET-HRP conjugate in the BO wells, the standard wells, and the sample wells.  Do NOT add HRP conjugate into the BL wells.
• Incubate the plate at room temperature for two hours.
• Wash the plate three times with 400 microliters of the diluted Wash Buffer per well.
• After the last of the three wash cycles pat the plate dry onto some paper toweling
• Add 200 microliters of the TMB substrate to all of the wells (including BL wells).
• Incubate the plate at room temperature for 15-30 minutes.
• Add 50 micoliters of 2 N sulfuric acid to all of the wells.
• Read the plate at 450 nm.

 

---